| 血清岩藻糖测定方法学研究 |
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| 引用本文： | 阎有功,解兵.血清岩藻糖测定方法学研究[J].中国生物化学与分子生物学报,1993,9(5):597-600. |
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| 作者姓名： | 阎有功 解兵 |
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| 作者单位： | 广州军区武汉总医院，广州军区武汉总医院 武汉 430070 广州第一军医大学医学实验技术大专班，武汉 430070 |
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| 摘 要： | 本文对血清岩藻糖测定方法进行了系统研究。血清用量由200μL减至50μL,显色反应4h内稳定。吸收峰在396nm。岩藻糖浓度40μg/mL内线性良好,批间CV=2.1％。以Sephadex-G200层析,血清岩藻糖主要存在于分子量为500kD的组分中。以本法测得30例健康人血清岩藻糖浓度为588.1±172.0μmol/L。
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| 关 键 词： | 血清 岩藻糖 |
| 收稿时间： | 1993-10-20 |
A Study of the Determination of Serum Fucose Concentration |
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| Yan,You-gong Xie,Bing.A Study of the Determination of Serum Fucose Concentration[J].Chinese Journal of Biochemistry and Molecular Biology,1993,9(5):597-600. |
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| Authors: | Yan You-gong Xie Bing |
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| Institution: | (Wuhan General Hospital of Guangzhou Army Area, 430070 |
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| Abstract: | We examined the biochemical properties of reaction products during the determination of serum fucose. The optical density at 396nm (A_(396)) of the reaction products increased linearly with the increase of fueose concentrations and was stable for at least 210—240min at room temperature. Sephadex G-200 gel chromatography of the mixed serum samples revealed a single peak of fucose in the void volume fraction of interest, suggesting that the free fucose in serum was mainly detected in the low-molecular weight fraction less than 66kD. |
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| Keywords: | Serum Fueose Determination |
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