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Polymorphic microsatellite loci in the Australian tree frog, <Emphasis Type="Italic">Litoria peronii</Emphasis>
Authors:Craig D H Sherman  Mats Olsson
Institution:(1) Institute for Conservation Biology, School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia
Abstract:Eleven novel polymorphic microsatellite loci developed from a microsatellite enriched genomic library, are presented for the Australian tree frog Litoria peronii. We screened 29 individuals from a single population and detected high levels of polymorphism for all 11 loci with the number of alleles/locus ranging from 9 to 24. Values of expected and observed heterozygosities ranged from 0.789–0.955 and 0.207–1.00, respectively. These microsatellite markers should prove useful in determining levels of genetic diversity, measuring gene flow and migration, assigning individuals to their most likely population of origin, and in the assignment of paternity.
Keywords:Microsatellite  Amphibian  Inbreeding  Paternity analysis  Genetic diversity
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