ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions |
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| Authors: | Roussakis Emmanuel Voutsadaki Styliani Pinakoulaki Eftychia Sideris Dionisia P Tokatlidis Kostas Katerinopoulos Haralambos E |
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| Affiliation: | aDepartment of Chemistry, University of Crete, Heraklion 71003, Crete, Greece;bInstitute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas (IMBB-FORTH), Heraklion 71110, Crete, Greece;cDepartment of Biology, University of Crete, Heraklion 71409, Crete, Greece;dDepartment of Materials Science and Technology, University of Crete, Heraklion 710 03, Crete, Greece |
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| Abstract: | ![]()
A new fluorescent Zn2+ indicator, namely, ICPBCZin was synthesized and the spectral profile of its free and Zn2+ bound forms was studied. The newly synthesized zinc indicator incorporates as chromophore the chromeno [3′,2′:3,4]pyrido[1,2a] [1,3]benzimidazole moiety and belongs to the dicarboxylate-type of zinc probes. The compound is excited with visible light, exhibits high selectivity for zinc in the presence of calcium and other common biological ions, and its Zn2+ dissociation constant is 4.0 nM. Fluorescence spectra studies of ICPBCZin indicated a clear shift in its emission wavelength maxima upon Zn2+ binding, as it belongs to the class of Photoinduced Charge Transfer (PCT) indicators, along with changes in fluorescence intensity that enable the compound to be used as a ratiometric, visible-excitable Zn2+ probe. |
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| Keywords: | Intacellular zinc Zinc ion sensor Ratiometric probe Fluorescent dye |
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