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| pta基因敲除对L-色氨酸发酵的影响 | |
| 引用本文： | 黄静,史建明,刘倩,徐庆阳,谢希贤,温廷益,陈宁. pta基因敲除对L-色氨酸发酵的影响[J]. 微生物学报, 2011, 51(4): 480-487 |
| 作者姓名： | 黄静 史建明 刘倩 徐庆阳 谢希贤 温廷益 陈宁 |
| 作者单位： | 1. 天津科技大学生物工程学院,教育部工业微生物重点实验室,天津,300457 2. 中国科学院微生物研究所,北京,100101 |
| 基金项目： | 国家“重大新药创制”科技重大专项课题(2008ZX09401-05)；“十一五”国家科技支撑计划重点项目(2008BAI63B01)资助。 |
| 摘 要： | [目的]探究pta基因缺失对大肠杆菌发酵生产L-色氨酸的影响.[方法]运用Red重组技术敲除pta基因,构建pta缺失株E.coli TRTH△pta.利用30 L发酵罐进行分批补料发酵试验,考察重组菌E.coliTRTHApta发酵0生产L-色氨酸过程中生物量、L-色氨酸产量、有机酸含量、发酵液中NH4+浓度及变化.... |
| 关 键 词： | Red重组技术 pta基因 基因敲除 乙酸 L-色氨酸 |
| 收稿时间： | 2010-11-03 |
| 修稿时间： | 2011-01-14 |
Effects of gene pta disruption on L-tryptophan fermentation |
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| Jing Huang,Jianming Shi,Qian Liu,Qingyang Xu,Xixian Xie,Tingyi Wen and Ning Chen. Effects of gene pta disruption on L-tryptophan fermentation[J]. Acta microbiologica Sinica, 2011, 51(4): 480-487 | |
| Authors: | Jing Huang Jianming Shi Qian Liu Qingyang Xu Xixian Xie Tingyi Wen Ning Chen |
| Affiliation: | College of Biological Engineering, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin 300457, China;College of Biological Engineering, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin 300457, China;College of Biological Engineering, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin 300457, China;College of Biological Engineering, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin 300457, China;College of Biological Engineering, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin 300457, China;Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;College of Biological Engineering, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin 300457, China |
| Abstract: | |
| Keywords: | Keywords: Red recombination pta gene gene knock-out acetate L-tryptophan |
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