(1) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P.R. China;(2) The Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan, 250012, P.R. China
Abstract:
Pseudomonas stutzeri SDM oxidized dl-lactic acid (25.5 g l-1) into pyruvic acid (22.6 g l-1) over 24 h. Both NAD+-independent d-lactate dehydrogenase and NAD+-independent l-lactate dehydrogenase were found for the first time in the bioconversion of lactate to pyruvate based on the enzyme activity assay and proteomic analysis. Jianrong Hao and Cuiqing Ma contributed equally to this work