Mapping and nucleotide sequence of a newHLA class II light chain gene,DQB3 |
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Authors: | Asako Ando Jun Kawai Masahiro Maeda Kimiyoshi Tsuji John Trowsdale Hidetoshi Inoko |
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Institution: | (1) Department of Transplantation II, Tokai University School of Medicine, Bohseidai, Isehara, 259-11 Kanagawa, Japan;(2) Imperial Cancer Research Fund, Lincoln's Inn Fields, WC2A 3PX London, UK |
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Abstract: | A genomic clone specifying a new HLA class II antigen β chain,DQB3, was isolated from a human genomic phage library using aDQB1 cDNA probe under low stringency conditions. Southern hybridization and nucleotide sequence analyses identified the β2 domain
exon (exon 3) with several deleterious mutations and the CP-TM-CY exon connecting peptide, transmembrane, and cytoplasmic
regions, (exon 4)], but the first, second, and fifth exons encoding the 5′ UT-leader, the β1 domain, and the 3′ UT domain
of normal β chains, respectively, were entirely missing. The nucleotide sequences of these two exons were distinct from those
of other class II β chain genes, but slightly more related to theDQB1 andDQB2 genes than to other class II genes. TheDQB3 sequence mapped betweenDQA2 andDQB1, 15 kb upstream fromDQA2, by analysis of overlapping cosmid clones. This mapping was supported by the fact thatTaq I,Msp I, andBam HIDQB3 polymorphisms were perfectly correlated with theDQA2 polymorphism and not with any polymorphisms in theDR orDQ subregion, suggesting the presence of a hot spot for recombination betweenDQB3 andDQB1.
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have
been assigned the accession number M26577. |
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