| 牙鲆GH基因第1外显子区微卫星标记与幼鱼生长性状的相关分析 |
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| 引用本文： | 倪静,尤锋,刘思思,吴志昊,徐冬冬,文爱韵,徐永立,张培军.牙鲆GH基因第1外显子区微卫星标记与幼鱼生长性状的相关分析[J].动物学杂志,2011,46(5):108-113. |
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| 作者姓名： | 倪静 尤锋 刘思思 吴志昊 徐冬冬 文爱韵 徐永立 张培军 |
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| 作者单位： | 1. 中国科学院海洋研究所 实验海洋生物学重点实验室 青岛266071
2. 青岛易邦生物工程有限公司 青岛266032 |
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| 基金项目： | 国家973项目(No. 2010CB126304),公益性行业(农业)科研专项经费项目(No. 200903046),鲆鲽类产业技术体系:苗种繁育项目科学家课题(No. nycytx-50); |
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| 摘 要： | 摘要：以牙鲆（Paralichthys olivaceus）一个养殖群体中的100个个体为研究材料，分析了其GH基因第1外显子区域中微卫星座位的多态性，同时对各基因型与其体重、体长等生长性状进行了关联性分析。结果表明，该外显子微卫星座位具有一定的多态性，共检测到3个等位基因(A、B、C)、5种基因型，这3个等位基因的频...
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| 关 键 词： | 牙鲆幼鱼 GH基因第1外显子 微卫星 遗传多态性 生长性状相关 |
| 收稿时间： | 1/7/2011 12:00:00 AM |
| 修稿时间： | 2011/4/26 0:00:00 |
Correlation Analysis of Microsatellite DNA Marker in the GH Exon 1 Region with Growth Traits of Juvenile Olive Flounder Paralichthys olivaceus |
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| NI Jing,YOU Feng,LIU Si-Si,WU Zhi-Hao,XU Dong-Dong,WEN Ai-Yun,XU Yong-Li and ZHANG Pei-Jun.Correlation Analysis of Microsatellite DNA Marker in the GH Exon 1 Region with Growth Traits of Juvenile Olive Flounder Paralichthys olivaceus[J].Chinese Journal of Zoology,2011,46(5):108-113. |
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| Authors: | NI Jing YOU Feng LIU Si-Si WU Zhi-Hao XU Dong-Dong WEN Ai-Yun XU Yong-Li and ZHANG Pei-Jun |
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| Institution: | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Yebio Bioengineering Co. Ltd. of Qingdao, Qingdao 266032, China;Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071;Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 |
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| Abstract: | In the GH exon 1 region, a microsatellite locus was detected in 100 juvenile samples from a cultured stock of olive flounder, Paralichthys olivaceus. The polymorphism of this locus and the genetic effect of genotypes on economic traits in this stock were analyzed. The results indicated that this microsatellite locus was polymorphic. Five genotypes and three allele were tested. The frequency values of A, B and C alleles were 0.339, 0.609 and 0.517, respectively. The observed heterozygosity and expected heterozygosity of this locus were 0.540 3 and 0.511 2.And the polymorphism information content and the effective allele number were 0.423 8 and 2.045 8, respectively. One-way ANOVA result showed that fish with genotype AC were heavier than those with other genotypes and C was an effective allele in flounder weight. |
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| Keywords: | Juvenile flounder Exon 1 in GH gene SSR Genetic diversity Association with growth traits |
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