Genetic characterization and fine mapping of a novel thermo-sensitive genic male-sterile gene tms6 in rice (Oryza sativa L.) |
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Authors: | Dong Sun Lee Li Juan Chen Hak Soo Suh |
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Institution: | (1) Institute of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, 712-749, Korea;(2) Rice Research Institute, Yunnan Agricultural University, Kunming, Yunnan, 650201, China;(3) College of Natural Resources, Yeungnam University, Gyeongsan, Gyeongbuk, 712-749, Korea |
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Abstract: | The application of genetic male sterility in hybrid rice production has great potential to revolutionize hybrid seed production
methodology. The two-line breeding system by using thermo-sensitive genic male sterility (TGMS) has been discovered and successfully
developed as a breeding strategy in rice. One TGMS gene was investigated by a spontaneous rice mutant line, Sokcho-MS, originated
from a Korean japonica variety. It was shown that Sokcho-MS is completely sterile at a temperature higher than 27°C and/or lower than 25°C during
the development of spikelets, but fertile at the temperature ranging from 25 to 27°C regardless of the levels of day-length.
Genetic analysis and molecular mapping based on SSR, STS and EST markers revealed that a single recessive gene locus involved
the control of genic male sterility in Sokcho-MS. By using an F2 mapping population derived from a cross between Sokcho-MS and a fertile indica variety Neda, the new TGMS gene, designated as tms6, was mapped primarily to the long arm of chromosome 5 of Oryza sativa at the interval between markers E60663 (2.0 cM) and RM440 (5.8 cM). Subsequently, tms6 was fine mapped to the interval between markers RM3351 (0.1 cM) and E60663 (1.9 cM). As tms6 appeared to be independent of other mapped TGMS genes in rice, the genetic basis of Sokcho-MS was further discussed. |
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