| 慢性缺氧大鼠肺血管c-myc及bcl-2基因表达研究 |
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| 引用本文： | 陶清国,张珍祥.慢性缺氧大鼠肺血管c-myc及bcl-2基因表达研究[J].中国组织化学与细胞化学杂志,1998(4). |
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| 作者姓名： | 陶清国 张珍祥 |
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| 作者单位： | 同济医科大学附属同济医院呼吸病研究室 |
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| 摘 要： | 应用免疫组织化学和Ｎｏｒｔｈｅｒｎ杂交方法，对慢性缺氧大鼠肺组织（主要是肺血管壁）原癌基因ｃ－ｍｙｃ和抗凋亡基因ｂｃｌ－２表达进行了研究。免疫组织化学观察提示，正常对照组大鼠肺血壁Ｃ－ｍｙｃ和Ｂｃｌ－２蛋白仅为弱阳性或不表达，慢性缺氧１、２周组大鼠肺血管壁这两种抗原表达比对照组显著增强，呈强阳性，Ｎｏｒｔｈｅｒｎ杂交结果表明：慢性缺氧１、２周组大鼠肺组织内ｃ－ｍｙｃ和ｂｃｌ－２的ｍＲＮＡ表达比对照组显著增加，以上结果提示，ｃ－ｍｙｃ及ｂｃｌ－２两种基因调节的细胞增殖与凋亡可能参与了慢性缺氧性肺动脉高压的发病进程。
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| 关 键 词： | 大鼠 缺氧 肺 c－myc bcl－2 |
STUDY OF EXPRESSION OF c myc AND bcl 2 GENES IN PULMONARY VESSELS OF RATS EXPOSED TO CHRONIC HYPOXIA |
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| Tao Qinguo,Zhang Zhenxiang Respiratory Disease Research Laboratory,Tongji Hospital,Tongji Medical University,Wuhan China.STUDY OF EXPRESSION OF c myc AND bcl 2 GENES IN PULMONARY VESSELS OF RATS EXPOSED TO CHRONIC HYPOXIA[J].Chinese Journal of Histochemistry and Cytochemistry,1998(4). |
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| Authors: | Tao Qinguo Zhang Zhenxiang Respiratory Disease Research Laboratory Tongji Hospital Tongji Medical University Wuhan China |
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| Institution: | Tao Qinguo,Zhang Zhenxiang Respiratory Disease Research Laboratory,Tongji Hospital,Tongji Medical University,Wuhan 430030 China |
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| Abstract: | Using immunohistochemical technique and Northern blot hybrid, the present study investigated the expression of c myc and bcl 2 genes in the lungs(mainly in pulmonary vessels) of rats exposed to chronic hypoxia for 1 and 2 weeks. The results were as follows: C myc and Bcl 2 antigens expressed weakly or not expressed in pulmonary vessels of control group, but they expressed strongly in pulmonary vessels of two hypoxic groups. Northern blot analysis indicated: The expression of c myc, bcl 2 mRNA was increased significantly in pulmonary vessels of two hypoxic groups respectively compared with control group. Our results suggest that the abnormal expression of c myc and bcl 2 genes might contribute to chronic hypoxic pulmonary hypertension. |
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| Keywords: | Hypoxia Lung c myc bcl 2 Rat |
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