Aging and diethylstilbestrol-induced aneuploidy in male germ cells: a transgenic mouse model |
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Authors: | Y Xiao A D Tates J J W A Boei A T Natarajan |
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Institution: | (1) Department of Radiation Genetics and Chemical Mutagenesis, LUMC, Leiden University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands, NL |
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Abstract: | The incidence of aneuploidy in male germ cells was evaluated by analyzing extra marker chromosome(s) signal(s) in round and/or
hook spermatids of transgenic mice. Two types of transgenic mice were used as models. The inserted foreign DNA (λ-gt10LacZ
shuttle vector and/or pSVc-myc plasmid) was located at the middle of the long arms of chromosome 2 (λ DNA) and/or chromosome 8 (c-myc). The number of marker chromosomes present could easily be detected after fluorescence in situ hybridization (FISH) in testicular
cells. The frequency of spontaneous aneuploidy of chromosome 2 was similar in round spermatids of lambda and λ-myc mice. Differential involvement of chromosomes 2 and 8 was observed in both round and hook spermatids. The frequency of spontaneous
aneuploidy in round spermatids was higher than that in hook spermatids. The frequency of aneuploidy of marker chromosomes
was significantly higher in older mice (2 years old) than in younger ones. Diethylstilbestrol (DES)-induced aneuploidy was
dose dependent, and was not influenced by the stage at which germ cells were treated with DES. These results demonstrate the
usefulness of a transgenic mouse model for the study of aneuploidy in germ cells.
Received: 5 August 1998 / Accepted: 27 August 1998 |
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