Quantification of S-adenosyl Methionine in Microbial Cell Extracts |
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| Authors: | Jens Olaf Krömer Elmar Heinzle Christoph Wittmann |
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| Institution: | (1) Biochemical Engineering, Saarland University, POB 151150, 66023 Saarbruecken, Germany |
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| Abstract: | A sensitive method for quantification of S-adenosyl methionine (SAM) in microbial cell extracts was developed and applied to Corynebacterium glutamicum. The method is based on SAM being completely hydrolyzed into 18O-homoserine when extracted in boiling H218O and thus can be clearly distinguished by GC-MS analysis from naturally labeled homoserine present in the cell extract. Additional
quantification of the total homoserine pool, representing both SAM and homoserine, via HPLC allows separate determination
of both metabolites.
Received 23 August 2005; Revisions requested 30 August 2005; Revisions received 26 September 2005; Accepted 31 October 2005 |
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| Keywords: | AdoMet C1 metabolism GC-MS intracellular methionine metabolome SAM |
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