Heat shock elements are involved in heat shock promoter activation during tobacco seed maturation |
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Authors: | Ralf Prändl Fritz Schöffl |
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Institution: | (1) Lehrstuhl für Allgemeine Genetik, Universität Tübingen, Biologisches Institut, Auf der Morgenstelle 28, D-72076 Tübingen, Germany |
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Abstract: | The soybean Gmhsp17.3-B heat shock promoter is developmentally regulated in transgenic tobacco, as indicated by the constitutive expression of a -glucuronidase reporter in seeds 16]. In this paper, we show that both the heat shock promoter-driven -glucuronidase activity and the mRNA of the endogenous Nthsp18P gene accumulate coincident with the onset of seed desiccation. Deletions of the soybean Gmhsp17.3-B promoter, encompassing the heat shock element (HSE)-containing regions, revealed a co-localization of sequences responsible for heat induction and developmental expression. Moreover, synthetic HSEs fused to a TATA box sequence had the potential to stimulate the developmental expression of a GUS reporter gene in seeds of transgenic plants. |
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Keywords: | glucuronidase reporter heat shock element heat shock promoter seed desiccation transgenic tobacco |
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