Production of nitric oxide and tumor necrosis factor-alpha by Smilacis rhizoma in mouse peritoneal macrophages |
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Authors: | Chung Hwan-Suck Shin Chang-Ho Lee Eon-Jeong Hong Seung-Heon Kim Hyung-Min |
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Institution: | Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul 130-701, South Korea. |
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Abstract: | Using mouse peritoneal macrophages, we have examined the mechanism by which, Smilacis rhizoma (SR) regulates nitric oxide (NO) production. When SR was used in combination with recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production. However, SR had no effect on NO production by itself. The increased production of NO from rIFN-gamma plus SR-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-kappaB). Furthermore, treatment of peritoneal macrophages with rIFN-gamma plus SR caused a significant increase in tumor necrosis factor-alpha (TNF-alpha) production. PDTC also decreased the effect of SR on TNF-alpha production significantly. These findings demonstrate that SR increases the production of NO and TNF-alpha by rIFN-gamma-primed macrophages and suggest that NF-kappaB plays a critical role in mediating these effects of SR. |
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Keywords: | Nitric oxide Nitric oxide synthase Macrophages Nuclear factor kappa B Tumor necrosis factor-α Interferon-γ Smilacis rhizoma Oriental medicine SR Smilacis rhizoma NO nitric oxide rIFN-γ recombinant interferon-γ PDTC pyrrolidine dithiocarbamate NF-κB nuclear factor kappa B TNF-α tumor necrosis factor-α NOS NO synthase LPS lipopolysaccharide ELISA enzyme-linked immunosorbent assay |
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