Identification of N5-methyl-N5-formyl-2,5,6-triamino-4-hydroxypyrimidine as a major adduct in rat liver DNA after treatment with the carcinogens, N,N-dimethylnitrosamine or 1,2-dimethylhydrazine |
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Authors: | D T Beranek C C Weis F E Evans C J Chetsanga F F Kadlubar |
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Institution: | 1. National Center for Toxicological Research (HFT-110), Food and Drug Administration, Jefferson, AR 72079 USA;2. University of Michigan-Dearborn, Dearborn, MI 48128 USA |
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Abstract: | Human Tamm-Horsfall urinary glycoprotein from an individual of the blood group Sd(a+) phenotype was tritium-labelled by treatment with galactose oxidase and sodium boro3H]hydride and was then digested with endo-beta-galactosidase. A series of dialysable, labelled fragments was released from which a pentasaccharide was isolated that strongly inhibited the agglutination of Sd(a+) red cells by human anti-Sda serum and hence contained the Sda determinant structure. Reduction, methylation analysis and sequential exo-glycosidase digestion established the structure of the pentasaccharide as: GalNAc beta(1 leads to 4)NeuAc(2 leads to 3)]Gal beta(1 leads to 4)GlcNAc beta(1 leads to 3)Gal |
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Keywords: | hplc high pressure liquid chromatography DMN N N -dimethyl-nitrosamine DMH 1 2-dimethylhydrazine 7-Me-G 7-methylguanine 3-Me-G 3-methylguanine 3-Me-A 3-methyladenine 1-Me-A 1-methyladenine 3-me-dC 3-methyldeoxycytidine deuterated dimethylsulfoxide |
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