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Determination of aflatoxin levels in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescence detection
Authors:SM Pearson  AAG Candlish  KE Aidoo  JE Smith
Institution:(1) School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, G4 OBA, Scotland;(2) Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, G1 1XW, Scotland
Abstract:A technique for the detection of aflatoxins in pistachio and cashew nuts using immunoaffinity column clean-up with HPLC and fluorescent detection is presented. Recoveries were in the range of 79–99% for pistachio samples artificially contaminated with 10 mgrg total aflatoxins kg–1 of food sample. For cashew samples recoveries ranged from 80–106%. This method is proposed as an accurate technique for aflatoxin detection in the range of mgrg aflatoxins kg–1 nuts.
Keywords:aflatoxin  immunoaffinity  HPLC  nuts
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