Diversity and biocontrol potential of endophytic fungi in Brassica napus |
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| Institution: | 1. State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China;2. Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;3. United States Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA, USA;1. University of Helsinki, Department of Forest Sciences, P.O. Box 27, FIN-00014, University of Helsinki, Finland;2. Viikki Metabolomics Unit, University of Helsinki, Department of Biosciences, P.O. Box 56, FIN-00014, University of Helsinki, Finland;1. Institute of Environmental Research (INFU), Department of Chemistry and Chemical Biology, Chair of Environmental Chemistry and Analytical Chemistry, TU Dortmund, Otto-Hahn-Strasse 6, D-44221 Dortmund, Germany;2. Department of Biochemistry, Indian Institute of Science (IISc), Bangalore 560012, Karnataka, India;1. Centro de Investigaciones de Fitopatología (CIDEFI), Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, 1900 La Plata, Buenos Aires, Argentina;2. Cerealicultura, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, Buenos Aires, Argentina;3. Laboratorio de Biología Funcional y Biotecnología (BIOLAB), Facultad de Agronomía de Azul, Universidad Nacional del Centro de la provincia de Buenos Aires, Argentina;4. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;5. Departamento de Ecosistemas Agroforestales, Escuela Técnica Superior del Medio Rural y Enología, Universidad Politécnica de Valencia, Spain;1. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran;2. Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia;1. School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Republic of Korea;2. Department of Agriculture, Abdul Wali Khan University Mardan, Mardan, Pakistan;3. Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan;4. UoN Chair of Oman⿿s Medicinal Plants & Marine Natural Products, University of Nizwa, 616 Nizwa, Oman |
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| Abstract: | This study was conducted to isolate endophytic fungi from oilseed rape (Brassica napus), to identify the fungal endophytes based on morphology and ITS (ITS1-5.8S rDNA-ITS2) sequences, and to evaluate their efficacy in suppression of the plant pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea. Selected endophytic fungal isolates were further tested for promoting growth of oilseed rape in potting experiments. A total of 97 endophytic fungal isolates were obtained from roots (35), stems (49) and leaves (13) of B. napus. Forty fungal species were identified and most species (80%) belong to Ascomycota. The species composition is highly diversified with Simpson’s diversity index reaching 0.959. Alternaria alternata is the dominant species accounting for 12.4% of the isolates. Twenty-four isolates exhibited antifungal activity against S. sclerotiorum in dual cultures on potato dextrose agar forming inhibition zones of 3–17 mm in width. The culture filtrates of Aspergillus flavipes CanS-34A, Chaetomium globosum CanS-73, Clonostachys rosea CanS-43 and Leptosphaeria biglobosa CanS-51 in potato dextrose broth exhibited consistent and effective suppression of oilseed rape leaf blight caused by S. sclerotiorum. Fusarium oxysporum CanR-46 was detected capable of production of volatile organic compounds highly inhibitory to S. sclerotiorum and B. cinerea. Moreover, A. alternata CanL-18, Fusarium tricinctum CanR-70 and CanR-71r, and L. biglobosa CanS-51 exhibited growth-promoting effects on oilseed rape. These results suggest that B. napus harbors diversified endophytic fungi, from which potential biocontrol agents against S. sclerotiorum and B. cinerea, and for promoting growth of B. napus can be screened. |
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| Keywords: | Endophytic fungi Volatile organic compounds Biological control |
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