Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis |
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| Authors: | Yuan-Li Wang Qing Li Jing Xie Ming ZhuWen-Juan Sun Lin He Qun Wang |
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| Affiliation: | School of Life Science, East China Normal University, Shanghai, China |
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| Abstract: | |
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| Keywords: | CRLs, Cullin-RING finger ligases Nedd8, neural precursor cell-expressed developmentally downregulated 8 CUL4, Cullin 4 qRT-PCR, quantitative real-time PCR UPP, the ubiquitin proteasome pathway E3, ubiquitin-ligases EST, expressed sequence tag HRP, horseradish peroxidase PBS, phosphate-buffered saline PFA, paraformaldehyde IHC, immunohistochemistry IF, immunofluorescence H& E, hematoxylin and eosin DAB, 3, 3&prime N-diaminobenzidine tertrahydrochloride DAPI, 6-diamidine-2-phenylidole-dihydrochlo-ride ORF, open reading frame RACE, rapid amplification of cDNA end SE, standard error GSP, gene-specific primer |
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