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Selection of reference genes as internal controls for gene expression in tissues of red abalone Haliotis rufescens (Mollusca,Vetigastropoda; Swainson, 1822) |
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| Authors: | Edgar A López-Landavery Amelia Portillo-López Cristian Gallardo-Escárate Miguel A Del Río-Portilla |
| Institution: | 1. Laboratorio de Genética, Departamento de Acuicultura, Centro de Investigación Científica y de Educación Superior de Ensenada, Carretera Tijuana-Ensenada N° 3918, Zona Playitas C.P 22860, Ensenada BC, Mexico;2. Facultad de Ciencias, Universidad Autónoma de Baja California, Km 103 Carretera Tijuana-Ensenada, C.P 22860, Ensenada BC, Mexico;3. Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P. O. Box 160-C, Concepción, Chile |
| Abstract: | |
| Keywords: | HKG housekeeping gene ACTB β-actin BGLU β-glucuronidase CY cyclophilin GAPDH glyceraldehyde 3-phosphate dehydrogenase HPRTI hypoxanthine phosphoribosyltransferase 1 RPL5 ribosomal protein L5 SDHA succinate dehydrogenase TUBB β-tubulin UBC ubiquitin-conjunction enzyme TCA tricarboxylic acid psu practical salinity units LiCl lithium chloride NTC no template control E gene efficiency value Cq cycle quantification RQ relative quantities R2 determination coefficient |
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