Identification of quantitative trait locus (QTL) linked to dorsal fin length from preliminary linkage map of molly fish, Poecilia sp. |
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| Authors: | Bun Poh Keong Siti Shapor Siraj Siti Khalijah Daud Jothi Malar Panandam Arina Nadia Abdul Rahman |
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| Affiliation: | 1. Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia;2. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia;3. Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang Selangor, Malaysia |
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| Abstract: | A preliminary linkage map was constructed by applying backcross and testcross strategy using microsatellite (SSR) markers developed for Xiphophorus and Poecilia reticulata in ornamental fish, molly Poecilia sp. The linkage map having 18 SSR loci consisted of four linkage groups that spanned a map size of 516.1 cM. Association between genotypes and phenotypes was tested in a random fashion and QTL for dorsal fin length was found to be linked to locus Msb069 on linkage group 2. Coincidentally, locus Msb069 was also reported as putative homologue primer pairs containing SSRs repeat motif which encoded hSMP-1, a sex determining locus. Dorsal fin length particularly in males of Poecilia latipinna is an important feature during courtship display. Therefore, we speculate that both dorsal fin length and putative hSMP-1 gene formed a close proximity to male sexual characteristics. |
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| Keywords: | μl, Microlitre BC, Backcross bp, Base pairs cM, Centimorgan DFL, Dorsal fin length F1, First filial generation F2, Second filial generation LG, Linkage group LOD, Logarithm of odds min, Minute n, Number ° C, Degree celsius P, Probability QTL, Quantitative Trait Loci R2, Coefficient of determination sp., Species SSRs, Microsatellites USA, United States of America |
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