DptR2, a DeoR-type auto-regulator,is required for daptomycin production in Streptomyces roseosporus |
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| Authors: | Feng Wang Ni-Ni Ren Shuai Luo Xiao-Xia Chen Xu-Ming Mao Yong-Quan Li |
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| Affiliation: | 1. Zhejiang University, College of Life Sciences, Hangzhou 310058, China;2. Hangzhou Huadong Medicine Group Biotechnology Institute Co., Ltd., Hangzhou 310011, China;3. Key Laboratory of Microbial Biochemistry and Metabolism Engineering of Zhejiang Province, Hangzhou 310058, China |
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| Abstract: | |
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| Keywords: | A, adenosine bp, base pair(s) C, cytidine cDNA, DNA complementary to RNA DBD, DNA binding domain DeoR, deoxyribose regulator DNase, deoxyribonuclease dNTP, deoxyribonucleoside triphosphate egfp, enhanced green fluorescent protein EMSA, electrophoretic mobility shift assay G, guanosine HPLC, high-performance liquid chromatography HRP, horseradish peroxidase IPTG, isopropyl β-d-thiogalactopyranoside kb, kilobase(s) or 1000 bp kD, kilodalton(s) Kyn, kynurenine LB, Luria&ndash Bertani (medium) NRPS, non-ribosomal peptide synthetase PAGE, PA-gel electrophoresis PBP, periplasmic-binding fold protein PEG, poly(ethylene glycol) RNase, ribonuclease rpm, revolutions per minute SDS, sodium dodecyl sulfate T, thymidine Thr, threonine Trp, tryptophan u, unit(s) UV, ultraviolet WT, wild type |
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