Significant association of interleukin-4 gene intron 3 VNTR polymorphism with susceptibility to knee osteoarthritis |
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Authors: | Serbulent Yigit Ahmet Inanir Akın Tekcan Ercan Tural Gokhan Tuna Ozturk Gorkem Kismali Nevin Karakus |
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Institution: | 1. Gaziosmanpasa University, Faculty of Medicine, Department of Medical Biology, Tokat, Turkey;2. Gaziosmanpasa University, Faculty of Medicine, Department of Physical Therapy and Rehabilitation, Tokat, Turkey;3. Ondokuz Mayis University, Faculty of Medicine, Samsun, Turkey;4. Ondokuz Mayis University, Faculty of Medicine, Department of Physical Therapy and Rehabilitation, Samsun, Turkey;5. Ankara University, Faculty of Veterinary Medicine, Department of Biochemistry, Ankara, Turkey |
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Abstract: | ObjectiveInterleukin-4 (IL-4) is a strong chondroprotective cytokine and polymorphisms within this gene may be a risk factor for osteoarthritis (OA). We aimed to investigate genotype and allele frequencies of IL-4 gene intron 3 variable number of tandem repeats (VNTR) polymorphism in patients with knee OA in a Turkish population.MethodsThe study included 202 patients with knee OA and 180 healthy controls. Genomic DNA was isolated and IL-4 gene 70 bp VNTR polymorphism determined by using polymerase chain reaction (PCR) with specific primers followed by restriction fragment length polymorphism (RFLP) analysis.ResultsOur result show that there was statistically significant difference between knee OA patients and control group with respect to IL-4 genotype distribution and allele frequencies (p = 0.000, OR: 0.20, 95% CI: 0.10–0.41, OR: 0.22, 95% CI: 0.12–0.42, respectively).ConclusionsOur findings suggest that there is an association of IL-4 gene intron 3 VNTR polymorphism with susceptibility of a person for development of knee OA. As a result, IL-4 gene intron 3 VNTR polymorphism could be a genetic marker in OA in a Turkish study population. This is the first association study that evaluates the associations between IL-4 gene VNTR polymorphism and knee OA. |
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Keywords: | IL-4 interleukin 4 OA osteoarthritis PCR polymerase chain reaction RFLP restriction fragment length polymorphism VNTR variable number tandem repeat IL-4R interleukin-4 receptor RA rheumatoid arthritis ESRD end-stage renal disease SLE systemic lupus erythematosus SM severe malaria MS multiple sclerosis |
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