Monoclonal Antibodies Specific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase |
| |
| Authors: | Sueyoshi Kuni; Kubo Yoshihiro; Yamagishi Kenji; Ogura Nagao; Ochiai Kuniyasu; Fukushima Kazuo; Ikeda Tadashi; Nakagawa Hiroki |
| |
| Institution: | 1Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University Matsudo, Chiba 271, Japan
2Department of Microbiology, Nihon University School of Dentistry at Matsudo Matsudo, Chiba 271, Japan |
| |
| Abstract: | Two monoclonal antibodies, 17(3)9 and 36(79)4, were preparedagainst nitrate reductase from Spinacia oleracea L. leaves.An enzyme-linked immunosorbent assay showed that 17(3)9, butnot 36(79)4, reacted more strongly to heat-denatured than nativeantigen. These antibodies inhibited NADH-nitrate reductase aswell as its various partial activities including reduced methylvilogen-nitrate reductase, reduced flavin mononucleotide-nitratereductase and NADH-cytochrome c reductase activities, but notNADH-ferricyanide reductase activity. Immunoblotting after electrophoreticseparation of nitrate reductase fragments obtained by Staphyrococcusaureus V8 protease digestion of native enzyme revealed thatthe two monoclonal antibodies bind to different epitopes locatedon the 28 kDa of the NADH-ferricyanide reductase domain. (Received October 2, 1987; Accepted June 9, 1988) |
| |
| Keywords: | |
| 本文献已被 Oxford 等数据库收录！ |
|
