Legumin antibodies recognize polypeptides in coated vesicles isolated from developing pea cotyledons |
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Authors: | D G Robinson K Balusek H Freundt |
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Institution: | (1) Plant Physiology Institute, University of Göttingen, Göttingen |
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Abstract: | Summary A highly enriched coated vesicle fraction has been isolated from cotyledons of developing pea seeds. This, and coated vesicles isolated from bovine brain as well as from bean leaves were subjected to SDS-PAGE followed by Western blotting with legumin antibodies. A distinct cross reaction with two polypeptides at around 60 kDa was seen, but only with the coated vesicles isolated from peas. Since legumin is synthesized as a 60 kDa precursor, but occurs as 40 and 20 kDa polypeptides in the protein body, we interpret our results as giving support to the idea that reserve proteins, like lysosomal proteins, are transported via coated vesicles.Abbreviations CV
coated vesicle
- DTT
dithiothreitol
- SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis |
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Keywords: | Coated vesicle Intracellular transport Legumin precursor Cotyledons Pisum sativum |
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