Production of pyrroloquinazoline alkaloid from leaf and petiole-derived callus cultures of <Emphasis Type="Italic">Adhatoda zeylanica</Emphasis> |
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Authors: | K?Jayapaul P?B?Kavi?Kishor Email author" target="_blank">K?Janardhan?ReddyEmail author |
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Institution: | (1) Department of Botany, Osmania University, 500 007 Hyderabad, India;(2) Department of Genetics, Osmania University, 500 007 Hyderabad, India |
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Abstract: | Summary Callus cultures of Adhatoda zeylanica Medicus were established from leaf and petiole explants. Accumulation of a bioactive pyrroloquinazoline alkaloid, vasicine,
in callus cultures was detected and confirmed by thin layer chromatography, electron-ionization mass spectra, 13C NMR and high-pressure liquid chromatography analysis. The mass of vasicine obtained from leaf-derived callus cultures was
found as 188 and this is comparable to that of the authentic sample. The retention time for leaf-derived extract was 10.065
and for the petiole-derived extract was 9.78 (authentic sample had 9.6 retention time) on high-performance liquid chromatography.
The mass and NMR spectra were compared with the spectra obtained from the authentic sample of vasicine. Different growth regulators
greatly influenced the growth of callus cultures. The accumulation of vasicine was more in leaf-derived callus grown on Murashige
and Skoog (MS) medium with 2.3 μM kinetin, and 4.5 μM 2,4-dichlorophenoxyacetic acid. This is the first report on in vitro production of a pharmacologically important compound vasicine and its characterization by mass spectrometry and 13C NMR studies from callus cultures of Adhatoda zeylanica. |
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Keywords: | Adhatoda zeylanica callus cultures pyrroloquinazoline alkaloid vasicine |
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