Molecular cloning and expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Lonicera japonica |
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| Authors: | Keji Jiang Yan Pi Rong Hou Hainian Zeng Zhuoshi Huang Zheng Zhang Xiaofen Sun Kexuan Tang |
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| Affiliation: | (1) State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&;D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Handan Road 220, Shanghai, 200433, People’s Republic of China;(2) The Department of Plant Biology, North Carolina State University, Raleigh, NC 27695-7612, USA;(3) Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU-Nottingham Plant Biotechnology R&;D Center, Institute of Systems Biology, Shanghai Jiao Tong University, Shanghai, 200030, P.R. China; |
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| Abstract: | In jasmonate biosynthetic pathway, allene oxide synthase (AOS, EC 4.2.1.92), which is a cytochrome P450 (CYP74A), catalyzes the first committed step. We herein cloned a novel cDNA from Lonicera japonica Thunb., named LjAOS (GenBank accession: DQ303120), which was homologous to other AOSs. Southern blot analysis revealed that it was a multi-copy gene. Real-time quantitative PCR analysis showed that LjAOS mRNA accumulated most abundantly in alabastrums, in which the content of chlorogenic acid (CA, the major important active ingredient indicator) was previously proven to be the highest. |
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| Keywords: | Allene oxide synthase Chlorogenic acid Honeysuckle Jasmonate LjAOS Lonicera japonica |
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