| 盾壳霉产生几丁质酶的条件研究 |
| |
| 引用本文： | 胡燕梅,李国庆.盾壳霉产生几丁质酶的条件研究[J].武汉生物工程学院学报,2006(2). |
| |
| 作者姓名： | 胡燕梅 李国庆 |
| |
| 作者单位： | 武汉生物工程学院生物工程系 湖北武汉430415(胡燕梅)，华中农业大学植保系 湖北武汉430070(李国庆) |
| |
| 摘 要： | 本实验采用摇瓶培养研究了盾壳霉(Coniothyrium minitans)产生几丁质酶的条件。改良的天然马铃薯葡萄糖培养基(mPDB)较合成培养基(SMCS)更适宜作为盾壳霉产生几丁质酶的基础培养基。添加9种不同碳源试验表明葡萄糖较适宜于盾壳霉产几丁质酶;氮源试验表明硝酸钾是产酶的较适宜氮源。盾壳霉形成几丁质酶的培养时间以15 d为佳,培养的适宜pH值为6.5。
|
| 关 键 词： | 盾壳霉 几丁质酶 产酶务件 |
Research of Culture Conditions for Production of Chitinase by Coniothyrium minitans |
| |
| HU Yanmei,LI guoqing.Research of Culture Conditions for Production of Chitinase by Coniothyrium minitans[J].Journal of Wuhan Bioengineering Institute,2006(2). |
| |
| Authors: | HU Yanmei LI guoqing |
| |
| Abstract: | Conditions for chitinase producted by the mycoparasite Coniothyrium minitans were optimized. The results showed both carbon and nitrogen sources greatly affected the enzyme production. Glucose was detected to be the best carbon source, while potassium nitrate was the best nitrogen source. A medium of modified potato dextrose broth containing 5 g/L glucose and 1 g potassium nitrate was formulated. Time course study revealed that the proper time for harvesting the enzyme was 15 days after incubation in the selected medium and the proper pH was 6. 5. |
| |
| Keywords: | Coniothyrium minitans chitinase culture conditions |
| 本文献已被 CNKI 等数据库收录！ |
