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Effect of 28-homobrassinolide treatment on nickel toxicity in Brassica juncea
Authors:M. Masidur Alam  S. Hayat  B. Ali  A. Ahmad
Affiliation:(1) Plant Physiology Section, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India
Abstract:
Plants of Brassica juncea L. cv. T-59 were supplied with 50 or 100 μM nickel (Ni50, Ni100) at 10 d after sowing (DAS), and sprayed with 28-homobrassinolide (HBR) at 20 DAS. The plants treated with Ni alone exhibited reduced growth, net photosynthetic rate, content of chlorophyll, and the activities of nitrate reductase (E.C.1.6.6.1) and carbonic anhydrase (E.C. 4.2.1.1) at observed 40 DAS, whereas, the contents of peroxidase (PER), catalase (CAT), and proline were increased. However, the spray of HBR partially neutralized the toxic effect of Ni on most of the parameters. Moreover, the treatment of HBR in association with either of the Ni concentration boosted the contents of PER and CAT in leaves and that of proline both in leaves and roots.
Keywords:carbonic anhydrase  catalase  chlorophyll  leaf  nitrate reductase  peroxidase  proline  protein  root  shoot
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