Using RAPDs to study phylogenetic relationships in Rosa |
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Authors: | T Millan F Osuna S Cobos A M Torres J I Cubero |
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Institution: | (1) Departamento de Genética, E.T.S.I.A.M., Aptdo. 3048, 14080 Córdoba, Spain;(2) Departamento de Mejora y Agronomia, C.I.D.A., Aptdo. 4240, 14080 Córdoba, Spain |
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Abstract: | Nineteen species of rose (Rosa sp.) were analysed using Random Amplified Polymorphic DNA markers (RAPD). Each 10-base-long arbitrary primer produced a specific DNA banding pattern that grouped plants belonging to the same species and botanical sections as predicted from their genetic background. One hundred and seventy-five amplification products were examined by cluster analysis to assess the genetic relationships among species and their genetic distances. All of the accessions belonging to 1 species grouped together before branching to other species. Dendrograms constructed for intra- and inter-specific studies showed a good correlation with previous classifications by different authors based on morphological and cariological studies. Our results show that the RAPD technique is a sensitive and precise tool for genomic analysis in rose, being useful in assigning unclassified accessions to specific taxonomic groups or else allowing accessions classified by traditional criteria to be re-classified. |
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Keywords: | Rosa sp RAPDs markers Polymerase chain reaction Phylogenetic relationships |
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