| 镉胁迫引起烟草悬浮细胞程序性死亡 |
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| 引用本文： | 支立峰,余涛,朱英国,李阳生.镉胁迫引起烟草悬浮细胞程序性死亡[J].武汉植物学研究,2006,24(5):403-407. |
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| 作者姓名： | 支立峰 余涛 朱英国 李阳生 |
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| 作者单位： | 1. 湖北师范学院生物系,湖北黄石,435002
2. 武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉,430072 |
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| 摘 要： | 镉胁迫会造成烟草悬浮细胞大规模死亡。通过TUNEL技术和琼脂糖凝胶电泳技术的检测发现,这种细胞死亡伴随有典型的DNA“梯形带”出现,表明这种由Cd胁迫引起的细胞死亡是一种程序性死亡。受胁迫细胞氧化性增强及细胞中丙二醛(MDA)水平升高,说明Cd胁迫时会在细胞中造成大量活性氧(ROS),暗示烟草细胞的程序性死亡可能与ROS有关。
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| 关 键 词： | 烟草 悬浮培养细胞 细胞程序性死亡(PCD) Cd胁迫 活性氧(ROS) |
| 文章编号： | 1000-470X（2006）05-0403-05 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006-03-162006-07-02 |
Programmed Cell Death of Tobacco Suspension Cultures Induced by Cd |
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| ZHI Li-Feng,YU Tao,ZHU Ying-Guo,LI Yang-Sheng.Programmed Cell Death of Tobacco Suspension Cultures Induced by Cd[J].Journal of Wuhan Botanical Research,2006,24(5):403-407. |
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| Authors: | ZHI Li-Feng YU Tao ZHU Ying-Guo LI Yang-Sheng |
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| Institution: | 1, Department of Biology, Hubei Normal University, Huangshi,Hubei 435002, China; 2, Key Laboratory of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University,Wuhan 430072, China |
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| Abstract: | Heavy metals can occur the large-scale cell death of tobacco suspension cultures.TUNEL study and genomic DNA gel electrophoresis showed that this kind of cell death was accompanied by PCD-specific DNA ladder.This result supports that cell death caused by Cd is a kind of programmed cell death(PCD).We have also found that the oxidative and the malondialdehyde(MDA) levels of the stressed cells were enhanced in PCD process,this phenomenon shows that the reactive oxygen species(ROS) in the stressed cell was increased.This suggests that the PCD of tobacco cells are tightly linked to the ROS. |
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| Keywords: | Tobacco Suspension cells Programmed cell death(PCD) Cd stress Reactive oxygen species(ROS) |
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