Relationship between fruiting body development and extracellular laccase production in the edible mushroom Flammulina velutipes |
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| Authors: | Aylin Cesur Ryousuke Yamamoto Yasuhiko Asada Akira Watanabe |
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| Affiliation: | 1. Department of Applied Bioresource Science, The United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790–8566, Japan;2. Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki-cho, Kagawa, 761–0795, Japan |
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| Abstract: | ![]()
The biochemical mechanism underlying the development of fruiting bodies in Flammulina velutipes, an edible mushroom, was investigated using the YBLB colorimetric assay to distinguish between the normal strain (FVN-1) and the degenerate strain (FVD-1). In this assay, the color of the YBLB medium (blue-green) inoculated with FVN-1 exhibiting normal fruiting body development changed to yellow, while the color of the medium inoculated with FVD-1 changed to blue. In this study, we found that this color difference originated from extracellular laccase produced by FVN-1. Moreover, FVN-1 exhibited considerably higher extracellular laccase activity than FVD-1, under conditions facilitating fruiting body formation. Overall, these findings suggest that extracellular laccase is involved in the fruiting body development process in F. velutipes. |
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| Keywords: | Fruiting body Laccase FVN-1" },{" #name" :" keyword" ," $" :{" id" :" kwrd0030" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" __text__" ," _" :" normal " },{" #name" :" italic" ," _" :" Flammulina velutipes" },{" #name" :" __text__" ," _" :" strain FVD-1" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" __text__" ," _" :" degenerate " },{" #name" :" italic" ," _" :" Flammulina velutipes" },{" #name" :" __text__" ," _" :" strain PDA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" potato dextrose agar BTB" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" bromothymol blue ABTS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
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