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Human and Extracellular DNA Depletion for Metagenomic Analysis of Complex Clinical Infection Samples Yields Optimized Viable Microbiome Profiles
Authors:Maria T Nelson  Christopher E Pope  Robyn L Marsh  Daniel J Wolter  Eli J Weiss  Kyle R Hager  Anh T Vo  Mitchell J Brittnacher  Matthew C Radey  Hillary S Hayden  Alexander Eng  Samuel I Miller  Elhanan Borenstein  Lucas R Hoffman
Institution:1. Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98105, USA;2. Department of Pediatrics, University of Washington School of Medicine, Seattle, WA 98105, USA;3. Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98105, USA;4. Department of Medicine, University of Washington School of Medicine, Seattle, WA 98105, USA;5. Department of Computer Science and Engineering, University of Washington School of Medicine, Seattle, WA 98105, USA;6. Medical Scientist Training Program, University of Washington School of Medicine, Seattle, WA 98105, USA;7. Child Health Division, Menzies School of Health Research, Charles Darwin University, Casuarina, NT 0811, Australia;8. Pulmonary and Sleep Medicine, Seattle Children’s Hospital, Seattle, WA 98105, USA;9. Blavatnik School of Computer Science, Tel Aviv University, Tel Aviv 6997801, Israel;10. Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel;11. Santa Fe Institute, Santa Fe, NM 87501, USA
Abstract:
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  • Keywords:cystic fibrosis  microbiome  metagenome  microbiota  human DNA  extracellular DNA  clinical samples  infection samples  polymicrobial infections  sputum
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