Enhancement of ribitol production during fermentation of <Emphasis Type="Italic">Trichosporonoides oedocephalis</Emphasis> ATCC 16958 by optimizing the medium and altering agitation strategies |
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Authors: | Liangzhi Li Huaxing Zhang Jiaolong Fu Chao Hu Yayue Zheng Yexian Qiu |
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Institution: | 1.School of Chemistry and Bioengineering,Suzhou University of Science and Technology,Suzhou,China;2.Jiangsu Key Laboratory for Environment Functional Materials,Suzhou University of Science and Technology,Suzhou,China;3.College of Biological and Chemical Engineering, Ningbo Institute of Technology,Zhejiang University,Ningbo,China |
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Abstract: | To maximize the productivity of ribitol, which is an important starting material for the production of one expensive rare
sugar, L-ribose, the effects of culture medium and agitation speed on cell growth as well as on the productivity of ribitol
were thoroughly investigated in a 7 L fermentor. The maximum volumetric productivity, 0.322 g/L/h of ribitol, were obtained
at an initial glucose concentration of 200 g/L in a batch culture. Based on the optimum glucose concentration, the ribitol
yield conversed from glucose was up to 0.193 g/g when 1% yeast extract was used as a nitrogen source. When the agitation speed
was maintained at 200 rpm, the ribitol concentration of 38.60 g/L was collected after 120 h of cultivation time. Additionally,
the scheme of two-phase agitation and glucose infusion was employed. To begin, in the first 24 h of fermentation, a high agitation
rate at 350 rpm and the initial glucose concentration of 50 g/L were applied, and the biomass concentration of 25.50 g/L was
achieved at 36 h of incubation; whereas this value was observed until 60 h in the former batch fermentation methods. Then,
in the second phase, with the agitation speed reduced to 150 rpm and the infusion amount of glucose controlled at 150 g/L,
the yield of ribitol reached to 65.00 g/L in two-phase agitation fermentation and was 1.68 fold of that obtained in one-stage
batch fermentation. To our knowledge, this study first demonstrates its significant effectiveness in improving ribitol production
with the application of Trichosporonoides oedocephalis ATCC 16958. |
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