Protective Effects of Piperine Against Corticosterone-Induced Neurotoxicity in PC12 Cells |
| |
Authors: | Qing-Qiu?Mao Zhen?Huang Email author" target="_blank">Siu-Po?IpEmail author Yan-Fang?Xian Chun-Tao?Che |
| |
Institution: | (1) School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong;(2) College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou, 310053, Zhejiang, China;(3) Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60612, USA; |
| |
Abstract: | Hyperactivation of the hypothalamic–pituitary–adrenal axis and the associated hippocampal atrophy were observed in patients
with depression, which could be ameliorated by the treatment with antidepressants. Therefore, neuroprotection has been proposed
to be one of the acting mechanisms of antidepressant. Our previous studies have showed that treating mice with piperine produced
antidepressant-like effect in animal models of behavioral despair. This study aimed to examine the protective effect of piperine
treatment on corticosterone-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells. The results showed that piperine
co-treatment revealed a differential effect on the cytotoxicity of corticosterone and had its maximum inhibitory effect at
1 μM. Piperine (1 μM) co-treatment also significantly decreased intracellular reactive oxygen species level, and enhanced
superoxide dismutase activity and total glutathione level in corticosterone-treated PC12 cells. In addition, piperine (1 μM)
co-treatment was found to reverse the decreased brain-derived neurotrophic factor (BDNF) mRNA level caused by corticosterone
in PC12 cells. The results suggest that piperine exerts a neuroprotective effect on corticosterone-induced neurotoxicity in
PC12 cells, at least in part, via the inhibition of oxidative stress and the upregulation of BDNF mRNA expression. This neuroprotective
effect may be one of the acting mechanisms accounts for the in vivo antidepressant activity of piperine. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|