家蝇转铁蛋白基因的克隆和生物信息学分析

为了研究家蝇转铁蛋白基因功能,获得全长cDNA序列并对其蛋白序列进行生物信息学分析,利用在线分析程序和相关工具软件分析转铁蛋白基因的开放读码框,分析编码蛋白的理化性质、结构域、并预测其空间结构和功能。结果表明家蝇转铁蛋白基因编码蛋白由622个氨基酸组成,分子量为70.58kDa,理论等电点为5.33,为稳定蛋白,有跨膜区,含有信号肽,该蛋白属于TR-FER保守结构域家族,亚细胞定位于细胞核,二级结构以无规则卷曲为主,功能预测该蛋白具有酶活性。

Cloning and bioinformatics analysis of transferrin gene of Musca domestica (Housefly)

To obtain and perform the bioinformatics analysis of Transferrin gene of Musca domestica（Housefly） so as to provide the basis for its＇ function research. Internet online procedures and the related software were exploited to analyze the open reading frame （ORF） of Transferrin gene, physical and chemical properties of Transferrin protein and the domains of the protein, and predict the space structure and functions of the protein. The Transferrin protein sequence was composed of 622 amino acids with 70.58 kDa of the molecular weight. Theoretical isoelectric point was 5.33. The protein was stable and had structures of transmembrane and signal peptide. The protein belonged to the family of TR - FER. The protein probably located in nucleus and dominated by random coils in second struc- ture. The ProtFun result showed the protein has enzymatic activity.