Establishing micropropagation conditions for five Cotoneaster genotypes

Micropropagation from shoot tips of five Cotoneaster genotypes C. franchetii, C dammeri var. Radicans, C. dammeri Skogholm, C. dammeri Eichholz N° 1 and C. dammeri Streib's Findling) is described. For culture initiation and multiplication, a medium based on Murashige and Skoog basal salts was suitable for all genotypes. For multiplication, 4.4 M 6-benzyladenine provided the optimum results, while auxins proved to be essential for rooting in vitro. However, direct acclimatization of unrooted microcuttings was more efficient than in vitro rooting and acclimatization of rooted plants.Abbreviations BA 6-benzyladenine - GA₃ gibberellic acid - IAA indole-3-acetic acid - IBA indole-3-butyric acid - MS Murashige & Skoog (1962) basal medium - NAA -naphthaleneacetic acid