Zinc induces unfolding and aggregation of dimeric arginine kinase by trapping reversible unfolding intermediate |
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Authors: | Liu Taotao Wang Xicheng |
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Affiliation: | Tsinghua University, Beijing, China. |
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Abstract: | Arginine kinase plays an important role in the cellular energy metabolism of invertebrates. Dimeric arginine kinase (dAK) is unique in some marine invertebrates. The effects of Zn2(+) on the unfolding and aggregation of dAK from the sea cucumber Stichopus japonicus were investigated. Our results indicated that Zn2(+) caused dAK inactivation accompanied by conformational unfolding, the exposure of hydrophobic surface, and aggregation. Kinetic studies showed the inactivation and unfolding of dAK followed biphasic kinetic courses. Zn2(+) can affect unfolding and refolding of dAK by trapping the reversible intermediate. Our study provides important information regarding the effect of Zn2(+) on metabolic enzymes in marine invertebrates. |
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