Expression of the <Emphasis Type="Italic">Apx</Emphasis> gene family during leaf senescence of <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
| |
Authors: | Irina?I?Panchuk Email author" target="_blank">Ulrike?ZentgrafEmail author Roman?A?Volkov |
| |
Institution: | (1) Department of Biochemistry, National University of Chernivtsy, Kotsubinsky str. 2, 58012 Chernivtsy, Ukraine;(2) ZMBP—Center of Molecular Biology of Plants, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany |
| |
Abstract: | Oxygen-free radicals are thought to play an essential role in senescence. Therefore, the expression patterns of the small
gene family encoding the H2O2 scavenging enzymes ascorbate peroxidase (APX; EC 1.11.1.11) were analyzed during senescence of Arabidopsis thaliana (L.) Heinh. Applying real-time RT-PCR, the mRNA levels were quantified for three cytosolic (APX1, APX2, APX6), two chloroplastic
types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms identified in the genome of Arabidopsis. The genes of chloroplastic thylakoid-bound tAPX and the microsomal APX4 exhibit a strong age-related decrease of mRNA level
in leaves derived from one rosette as well as in leaves derived from plants of different ages. In contrast to the tAPX, the
mRNA of sAPX was only reduced in old leaves of old plants. The microsomal APX3 and APX5, and the cytosolic APX1, APX2, and
APX6 did not show remarkable age-related changes in mRNA levels. The data show that expression of the individual APX genes
is differentially regulated during senescence indicating possible functional specialization of respective isoenzymes. The
hydrogen peroxide levels seem to be controlled very precisely in different cell compartments during plant development. |
| |
Keywords: | Actin2 Arabidopsis Ascorbate peroxidase Leaf senescence Real-time RT-PCR |
本文献已被 PubMed SpringerLink 等数据库收录! |
|