Increases of Calcium,Phosphorus, and Magnesium in Both the Right and Left Fibrous Trigones of Human Heart with Aging |
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| Authors: | Setsuko Tohno Cho Azuma Yoshiyuki Tohno Yumi Moriwake Atsuko Izu Tsukasa Kumai Takeshi Minami |
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| Institution: | (1) Department of Medical Research and Education, Nephrology Laboratory, Taipei Veterans General Hospital, Taipei, Taiwan, ROC;(2) Department of Medical Research and Education, Biophysics Laboratory, Taipei Veterans General Hospital, Taipei, Taiwan, ROC;(3) Department of Medical Research and Education, Metabolism Laboratory, Taipei Veterans General Hospital, Taipei, Taiwan, ROC;(4) Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan, ROC;(5) Department of Medical Research and Education, Taipei Veterans General Hospital, Shih-Pai, Taipei, Taiwan, 11217, ROC |
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| Abstract: | We previously reported that reduced platelet endogenous antioxidant enzymes activities are related to the low plasma zinc
level in patients with end-stage renal failure (ESRF). In this study, we attempt to evaluate whether dietary zinc deprivation
reduces the activities of endogenous antioxidant and then enhances oxidative stress in the unstimulated platelet of normal
and 5/6 nephrectomized (Nx) rats because increased platelet oxidative stress is suggested to involve in the incidence of thrombotic
and atherosclerotic diseases. Male Sprague–Dawley rats (n = 48) were fed a zinc-deficient diet and deionized distilled water for 1 week to induce reduction of plasma zinc level. Half
of the rats continued on this diet for 4 weeks as zinc-deplete group, and the other half were maintained on the same diet
but with zinc-supplemented water (120 mg/L zinc sulfate solution) to correct the reduction of plasma zinc level as zinc-replete
group. Half of each group underwent 5/6 Nx, while the other half underwent sham operation. Another 12 normal rats were fed
standard rat chow (containing 23.4% protein and 50 ppm zinc) and drank deionized distilled water as normal control rats. In
zinc-deplete rats including sham-operated and 5/6 Nx rats exhibited lower endogenous antioxidant enzymes activities such as
reduced glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GPX) and higher malondialdehyde (MDA) levels
than normal control rats in the unstimulated platelets. However, in zinc-replete rats including sham-operated and 5/6 Nx rats
have a normal endogenous antioxidant enzymes activity and normal MDA levels in the unstimulated platelets. We suggest that
in uremia, the low plasma zinc level may be a risk factor for thrombotic and atherosclerotic diseases because it reduces the
activities of endogenous antioxidant enzymes and increases oxidative stress in the unstimulated platelet.
Supported by grant 92-117 from Taipei Veterans General Hospital |
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| Keywords: | Chronic renal failure Zinc Platelet Oxidative stress |
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