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Microdissection and microcloning of the long arm of human chromosome 7 |
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| Authors: | R. Kaiser J. Weber K. -H. Grzeschik J. E. Edström A. Driesel S. Zengerling M. Buchwald L. C. Tsui K. Olek |
| Affiliation: | (1) Inst. f. Strahlenbiologie Univ. Bonn, Sigmund-Freud-Str. 15, 5300 Bonn 1, Germany;(2) Inst. f. Humangenetik Univ. Münster, Münster, Germany;(3) Inst. of Genetics, Univ. of Lund, Sweden;(4) DECHEMA-Institut, Frankfurt, Germany;(5) Hospital For Sick Children, Toronto, Canada |
| Abstract: | DNA-fragments from the region of the long arm of human chromosme 7 to which the CF-locus has been mapped recently were isolated by microdissection and microcloning. We developed a new fixation procedure resulting in inserts of 1.0–7.0 kb in length with a mean value of 2.9 kb. Regional mapping of three clones on 7q was carried out by the use of different hybrid cell lines containing fragments of human chromosome 7. |
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