The development and application of a radioimmunoassay for 18-hydroxy-corticosterone. |
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| Authors: | V I Martin C R Edwards E G Biglieri G P Vinson F C Bartter |
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| Affiliation: | 1. The Department of Chemical Pathology and the Medical Professorial Unit, St. Bartholomew''s Hospital, London, EC1A 7BE UK;2. San Francisco General Hospital, University of California U.S.A.;3. Department of Zoology, St. Bartholomew''s Medical College, Charterhouse Square, London, EC1A 7BE UK;11. Endocrinology Division, National Heart and Lung Institute, National Insititutes of Health, Bethesda, Maryland U.S.A. |
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| Abstract: | ![]()
A sensitive and specific radioimmunoassay has been developed for 18-hydroxy-corticosterone (18-OH-B) and applied to the measurement of this steroid in peripheral plasma. High specific activity label (3H-18-OH-B) was prepared using the incubation of 3H-corticosterone with duck adrenal mitochondria. Antisera were produced by immunisation with 18-OH-B gamma-lactone 3-oxime conjugated to bovine serum albumin. The antibodies examined showed 100% cross-reactivity with 18-hydroxy-deoxycorticosterone gamma-lactone (18-OH-DOC gamma-lactone), but minimal cross-reactivity with other steroids. Paper chromatography was used to separate 18-OH-DOC gamma-lactone from 18-OH-B gamma-lactone. The interassay precision was 7.6% and the intra-assay precision 11.0%. The accuracy of the method was confirmed by showing a linear relationship between amounts of 18-OH-B added and amounts of 18-OH-B gamma-lactone measured (y = 0.854 X +15.1, r = 0.9. p less than 0.001). The mean plasma level in normal subjects on an ad libitum sodium intake was 225 +/- 92.7 (SD) pg/ml (n = 17) when standing, and 99 +/- 38.3 (SD) pg/ml (n = 6) after lying down for 30 minutes. |
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