Thidiazuron stimulates adventitious shoot regeneration in different safflower explants |
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| Authors: | K Radhika M Sujatha T Nageshwar Rao |
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| Institution: | (1) Acharya N.G. Ranga Agricultural University, Rajendranagar, Hyderabad, 500030, India;(2) Directorate of Oilseeds Research, Rajendranagar, Hyderabad, 500030, India |
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| Abstract: | Adventitious shoot regeneration from root, hypocotyl, cotyledon and primary leaf explants of safflower (Carthamus tinctorius L.) was studied. Shoot regeneration was promoted by benzyladenine (BA) + naphthaleneacetic acid (NAA), BA + indole-3-butyric
acid (IBA), kinetin + NAA and thidiazuron (TDZ) + NAA incorporated in Murashige and Skoog (MS) basal medium. High frequency
of shoot regeneration and high number of shoots per regenerating explant were obtained on a wide range of TDZ + NAA combinations.
Proliferated shoots were elongated in MS + 0.5 mg dm−3 kinetin and well-developed shoots were rooted in half strength MS + 0.5 mg dm−3 NAA. Rooted shoots were successfully acclimatized and established in soil. |
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| Keywords: | auxins Carthamus tinctorius L cytokinins in vitro culture |
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