Construction of a cosmid library of Spirulina platensis as an approach to DNA physical mapping |
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Authors: | Edda De Rossi Giovanna Riccardi Anna Maria Sanangelantoni Orio Ciferri |
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Institution: | Dipartimento di Genetica e Microbiologia 'A. Buzzati Traverso', Universitàdi Pavia, Pavia, Italy |
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Abstract: | Abstract A Spirulina platensis gene library has been constructed using cosmid vector pMMB34. The cosmid bank was controlled for its random gene distribution by colony hybridization. Genes were identified using either homologous or heterologous probes of genes involved in photosynthesis (large and small subunit of d -ribulose 1,5-bisphosphate carboxylase, 32 kDa thylakoid protein, α, β subunits of C-phycocyanin) and protein synthesis (elongation factors EF-Tu, EF-G). |
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Keywords: | Spirulina platensis cosmid bank colony hybridization Southern blotting |
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