中国小球藻病毒及其分子生物学性质

以小球藻NC64A株系为寄主，从17个省市采集的上百个水样中分离到了11个病毒分离物（BJ－1、BJ－2、BJ－3、BJ－4、FJ－1、FJ－2、NJ－1、HCJ－1、CDT－1、SCB—1、SCC－1）。这些病毒具有许多相同的性质，如均为球形多面体，基因组为300kb的dsDNA。但它们的DNA限制性酶切图谱，碱基组成和蛋白组分等均有差异。FJ－1的主要外壳蛋白的分子量小于54000，其它10个分离物则与PBCV-1一样，主要外壳蛋白的分子量为54000。Westernblot分析的结果显示，除FJ－1外其它病毒分离物与PBCV—1的抗血清有较强的免疫交叉反应。说明这些病毒与PBCV－1的同源性较高。在11个病毒分离物中，FJ－1在蛋白组分、碱基组成等方面与其它分离物和PBCV—1差别较大。

CHINESE CHLORELLA VIRUSES AND THEIR MOLECULAR BIOLOGICAL PROPERTIES

An extensive survey for Chlorella viruses revealed that Chlorella viruses are widely distributed over China. Eleven isolates of Chlorella viruses have been isolated, which lysis the Chlorella viruses sp. (strain NC64A). These isolates were named BJ-1, BJ-2, BJ-3, BJ-4, FJ-1, FJ-2, NJ-1, CDT-1, HCJ-1, SCB-1 and SCC-1 respectively. They have several common properties, including polyhedral morphology, and linear dsDNA genomes. However, the restriction patterns of viral DNA, the concentration of m5dC and m6dA in viral DNA, and the composition of viral structure protein are different among these isolates. All of viral major capsid proteins are 54,000 except FJ-1, whose major capsid protein is smaller than 54,000. Western blot analysis showed strong immunological cross reaction among all of viral proteins with PBCV-1's antiserum except FJ-1. It indicates that the homology between these isolates and PBCV-1 is high. Among these isolates, FJ-1 has some special properties.