应用固定化里氏木霉糖化玉米秆纤维素的研究

采用多孔聚酯材料固定里氏木霉（TrichodermareeseiRutC30）菌丝细胞，将固定化细胞在生长限制条件下重复分批培养，使纤维酶的合成与玉米秆纤维原料的酶解糖化耦合在一个反应器中同时进行。在30℃、初始pH4.8、摇瓶转速150r／min的条件下，连续重复进行12次分批培养试验。每批玉米秆用量为60g／L，培养周期4．5d，共54d。培养液中含滤纸酶活力平均为0.70IU／ml，还原糖26．41g／L，糖化率达到理论值的89.11％。固定化菌丝形态正常，菌量保持在10g／L左右。在间歇添料条件下，玉米秆原料的总量可提高到120g／L，7d后还原糖浓度达52.81g／L，糖化率为89．20％。利用固定化里氏木霉同时产酶和糖化植物纤维原料，工艺简便、成本低廉、易于连续自动化操作，是一条有效利用可再生纤维素资源的新途径。

SACCHARIFICATION OF CORN STOVER BY IMMOBILIZED TRICHODERMA REESEI CELLS

The mycelia of Trichoderma reesei Rut C30 were adsorbed and immobilized within the porous polyurethane supporter. It was found that the synthesis of cellulase by the immobilized cells and the enzymatic hydrolysis of corn stover were able to be carried out in a nitrogen source-limiting medium. Under repeated batch processes with 60 g/L corn stover pretreated by 2% NaOH at 85 degrees C, the average cellulase activity (FPA) was 0.70 IU/ml, the concentration and yield of the reducing sugar were 26.41 g/L and 89.11% respectively after shaking culture at 150 r/min, 30 degrees C, pH 4.8 for 108 h. Total of 12 repeated batches were performed in 54 days. The immobilized cells kept the weight around 10 g/L. The fed-batch process was also studied with the same immobilized cells. Total of 120 g/L corn stover was added in different feeding manners. The reducing sugar of 52.8 g/L was produced after 7 days and the saccharification efficiency (89.2%) was almost the same as the batch process. The results indicated that the cellulase production and cellulosic material saccharification in situs by the immobilized Trichoderma reesei cells is an convenient and effective process for conversion and utilization of renewable biomass.