Somatic embryogenesis and plant regeneration in oil palm using the thin cell layer technique |
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Authors: | Jonny E Scherwinski-Pereira Rodrigo S da Guedes Jr" target="_blank">Paulo César P FerminoJr Tatiane L Silva Frederico Henrique S Costa |
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Institution: | 1.Embrapa Genetic Resources and Biotechnology,Laboratory of Tissue Culture and Plant Conservation,Brasília,Brazil;2.Department of Plant Science,Federal University of Acre,Rio Branco,Brazil;3.Department of Biotechnology,Federal University of Amazonas,Manaus,Brazil;4.Department of Plant Science,Federal University of Lavras,Lavras,Brazil |
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Abstract: | An efficient procedure has been developed for inducing somatic embryogenesis and regeneration of plants from tissue cultures
of oil palm (Elaeis guineensis Jacq.). Thin transverse sections (thin cell layer explants) of different position in the shoot apex and leaf sheath of oil
palm were cultivated in Murashige and Skoog (MS) (Physiol Plant 15:473–497, 1962) medium supplemented with 0–450 μM picloram and 2,4-D with 3.0% sucrose, 500 mg L−1 glutamine, and 0.3 g L−1 activated charcoal and gelled with 2.5 g L−1 Phytagel. Embryogenic calluses were evaluated 12 wk after inoculation. Picloram (450 μM) was effective in inducing embryogenic
calluses in 41.5% of the basal explants. Embryogenic calluses were maintained on a maturation medium composed of basal media,
plus 0.6 μM NAA and 12.30 μM 2iP, 0.3 g L−1 activated charcoal, and 500 mg L−1 glutamine, with subcultures at 4-wk intervals. Somatic embryos were converted to plants on MS medium with macro- and micronutrients
at half-strength, 2% sucrose, and 1.0 g L−1 activated charcoal and gelled with 2.5 g L−1 Phytagel. |
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