High-performance liquid chromatographic separation and isolation of quinidine and quinine metabolites in rat urine |
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Authors: | Susan E Barrow AA Taylor EC Horning MG Horning |
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Institution: | Institute for Lipid Research, Baylor College of Medicine, Houston, Texas 77030 U.S.A. |
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Abstract: | A procedure for the separation and isolation of the urinary metabolites of quinidine and quinine by reversed-phase high-performance liquid chromatography is described. Nine metabolites of quinidine and eight metabolites of quinine were detected in the urine of male Sprague-Dawley rats after a single dose of quinidine or quinine (50 mg kg?1). Following extraction from urine, the metabolites were separated on either an analytical or a semi-preparative reversed-phase column by gradient elution. After isolation and derivatization, the metabolites were analyzed by gas chromatography and gas chromatography—mass spectrometry. |
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