异麦芽低聚糖对衰老模型小鼠肠黏膜免疫功能调节作用的研究

目的探讨异麦芽低聚糖（Isomalto oligosaccharide，IMO）对衰老模型小鼠肠黏膜免疫功能的调节作用及可能机制。方法昆明纯系小鼠随机分为Young组、Aging组、IMO组和IMOLCM组。采用D-半乳糖造成衰老模型后，给予相应药物干预。采用细菌定量测定法检测肠道菌群、放射免疫法检测肠黏膜sIgA、免疫组化法检测肠黏膜IgA^＋浆细胞的表达。结果与Young组相比，Aging组小鼠存在肠道菌群失调、肠黏膜sIgA含量降低、IgA^＋浆细胞表达减少（P〈0．05）；与Aging组相比，IMO组肠道菌群失调状况有所改善，肠黏膜sIgA含量增加、IgA^＋浆细胞的表达增加（P〈0．05）；与IMO组相比，IMOLCM组肠道菌群失调再次出现，肠黏膜sIgA降低、IgA^＋浆细胞的表达降低（P〈0．05）。结论异麦芽低聚糖可改善衰老模型小鼠肠道菌群失调状态和提高肠黏膜免疫功能；异麦芽低聚糖提高肠黏膜免疫功能可能主要由增加益生菌数量间接实现的。

Effect of isomalto oligosaccharide on intestinal mucosal immunity of aging model mice

Objective To explore the modulating function of isomalto oligosaccharide on intestinal mucosal immunity of aging model mice and its possible mechanisms.Method 48 Kunming mice were randomly divided into 4 group: young control group,aging model group,IMO group and IMOLCM group.After the aging models were made by injection of D-galactose,the IMO group was given IMO while IMOLCM group was given IMO and lincomycin.Each group were killed after 30 days.We detected the quantity of intestinal flora by the method of quantitative detection;the level of sIgA of intestinal mucous membrane by the method of radioimmunity;the expression of the IgA+ plasma cell in mice′s intestinal mucous membrane by the immunohistochemical method.Result(1) Dysbacteria could be observed in aging mice;The level of sIgA of intestinal mucous membrane,IgA+ plasma cell in aging model group were lower than the young control group′s;(2) Dysbacteria had improwed in IMO group;The level of sIgA of intestinal mucous membrane,the number of IgA+ plasma cell in IMO group were higher than the aging model group′s(P<0.05);(3) Dysbacteria could be observed again in IMOLCM group;The level of sIgA of intestinal mucous membrane,the number of IgA+ plasma cell in IMOLCM group were lower than the IMO group′s(P<0.05).Conclusion(1) IMO can improve dysbacteriosis of aging model mice and enhance the function of intestinal mucosal immunity.(2) That IMO can improve immunity may come true mainly by increasing the number of probiotics.