Curcuminoid derivatives enhance telomerase activity in an in vitro TRAP assay |
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| Affiliation: | 1. Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA;2. Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA;3. Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA;4. Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA;1. Cardiovascular and Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health;2. Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health;3. Office of Biostatistics Research, Division of Cardiovascular Science, National Heart, Lung, and Blood Institute, National Institutes of Health;4. Department of Dermatology, Uniformed Services University of the Health Sciences, Bethesda, MD |
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| Abstract: | The length of telomeres controls the life span of eukaryotic cells. Telomerase maintains the length of telomeres in certain eukaryotic cells, such as germline cells and stem cells, and allows these cells to evade replicative senescence. Here, we report for the first time a number of curcuminoid derivatives that enhance telomerase activity in an in vitro TRAP assay. A preliminary analysis of structure–activity relationships found that the minimal requirement for this enhanced telomerase activity is a curcuminoid core with at least one n-pentylpyridine side chain, while curcuminoids with two such side chains exhibit even greater activity. The finding here might lead to a new class of telomerase activators that act directly or indirectly on telomerase, rather than through the reactivation of the telomerase reverse transcriptase (TERT) gene associated with other telomerase activators found in the literature. |
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| Keywords: | Telomerase Telomerase activator Cell senescence Curcuminoid derivatives |
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