Novel dammarane saponins from Gynostemma pentaphyllum and their cytotoxic activities against HepG2 cells |
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| Institution: | 1. Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;2. Laboratory of Comparative Medicine, Guangdong Medical Laboratory Animal Center, Foshan, Guangdong 528248, China;1. Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR China;2. The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, PR China;1. School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, People’s Republic of China;2. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People’s Republic of China;3. Shenyang Pharmaceutical University, Shenyang 110016, People’s Republic of China;1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China;2. Department of Emergency Medicine, Laboratory of Rescue Center of Severe Wound and Trauma, PLA, General Hospital of Shenyang Military Command, Shenyang 110016, PR China;3. Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China;1. CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics (LICP), Chinese Academy of Sciences (CAS), Lanzhou, 730000, PR China;2. Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian, 271000, PR China;3. Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai, 264010, PR China;4. Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran;1. Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;2. Department of Breast Disease Prevention Center, Haidian Maternal and Child Health Hospital, Beijing 100080, China |
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| Abstract: | Two new dammarane saponins, 2α,3β,12β-trihydroxydammar-20(22),24-diene-3-O-β-d-glucopyranoxyl(1→2)-β-d-6″-O-acetylglucopyranoside (1, namely damulin C) and 2α,3β,12β-trihydroxydammar-20(21),24-diene-3-O-β-d-glucopyranoxyl(1→2)-β-d-6″-O-acetylglucopyranoside (2, namely damulin D), were isolated from the ethanol extract of Gynostemma pentaphyllum, which had been heat processed by steaming at 125 °C. The NMR spectroscopic data of the novel saponins were completely assigned by using a combination of 2D NMR experiments including 1H–1H COSY, HSQC, and HMBC. Their cytotoxic activities of human liver adenocarcinoma HepG2 cells were evaluated in vitro. They showed cytotoxicities against HepG2 cell line with IC50 of 40 ± 0.7 and 38 ± 0.5 μg/ml, respectively. |
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| Keywords: | Dammarane saponin Damulin C Damulin D HepG2 cell cytotoxic activity |
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