Kinetics and physico-chemical characterization of exopolysaccharides produced by the cyanobacterium Oscillatoria formosa |
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Authors: | Jindal N Singh D P Khattar J I S |
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Institution: | (1) Department of Botany, Punjabi University, Patiala, 147 002, India; |
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Abstract: | Exopolysaccharides of the cyanobacterium Oscillatoria formosa have been physico-chemically characterized and kinetics of their production studied. The organism produced 334.8 μg EPS per
ml culture in 24 days with the maximum rate of production obtained during initial days of growth. HPLC analysis of the EPS
hydrolysate revealed that besides three unidentified sugars, EPS contained ribose, mannose, and galacturonic acid. FT-IR spectrum
of EPS revealed the presence of methyl, carboxyl and C–N groups. Elemental analysis indicated the presence of 4.7% nitrogen
in EPS. The organism produced 75.6% more EPS when incubated at 35°C compared to cultures at 28°C. Under varied nutritional
conditions, though the growth of the organism was less yet it produced enhanced amounts of EPS. Aqueous dispersions of EPS
showed non-Newtonian, pseudoplastic behaviour. The viscosity of the aqueous solution of EPS was quite stable over a wide range
of pH and temperature but it was observed to be affected by CaCl2. |
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